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ProductsBack/LYPLA2 antibody [N1C3]

LYPLA2 antibody [N1C3]

GTX102240
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetLYPLA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    LYPLA2 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11313
  • Target name
    LYPLA2
  • Target description
    lysophospholipase 2
  • Target synonyms
    acyl-protein thioesterase 2; APT2; APT-2; DJ886K2.4; LPL-II; lysophospholipase II; lysoPLA II; testicular tissue protein Li 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95372
  • Protein Name
    Acyl-protein thioesterase 2
  • Scientific Description
    Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. There are alternatively spliced transcript variants described for this gene but the full length nature is not known yet. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LYPLA2 using anti-LYPLA2 antibody (A10621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA2 antigen affinity purified polyclonal antibody (Catalog # A10621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA2 at approximately 25 kDa. The expected band size for LYPLA2 is at 25 kDa.
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