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LYVE1 antibody

20R-2482
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    LYVE1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA561582(LYVE1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of LYVE1 using anti-LYVE1 antibody (M04027). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYVE1 antigen affinity purified monoclonal antibody (Catalog # M04027) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYVE1 at approximately 29 kDa. The expected band size for LYVE1 is at 35 kDa.
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Flow cytometric analysis of Huvec cells with LYVE1 (5C1) Monoclonal Antibody (bsm-52811R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
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