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Macrophage + Monocyte antibody [MAC387]

GTX76577
GeneTex
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetS100A9
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Overview

  • Supplier
    GeneTex
  • Product Name
    Macrophage + Monocyte antibody [MAC387]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1/100-1/200. IHC-Fr: 1/100-1/200. FACS: 1/50-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MAC387
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6280
  • Target name
    S100A9
  • Target description
    S100 calcium binding protein A9
  • Target synonyms
    60B8AG; CAGB; calgranulin B; calprotectin L1H subunit; CFAG; CGLB; L1AG; leukocyte L1 complex heavy chain; LIAG; MAC387; MIF; migration inhibitory factor-related protein 14; MRP14; MRP-14; NIF; P14; protein S100-A9; S100-A9
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Fatal toxoplasmosis in a captive squirrel monkey (Saimiri boliviensis) in Portugal.
    Read more
  • Inflammation and lung injury in an ovine model of fluid resuscitated endotoxemic shock. Passmore MR et al., 2018 Nov 22, Respir Res
    Read more
  • Differential contribution of immune effector mechanisms to cortical demyelination in multiple sclerosis. Lagumersindez-Denis N et al., 2017 Jul, Acta Neuropathol
    Read more
  • Inflammation and lung injury in an ovine model of extracorporeal membrane oxygenation support. Passmore MR et al., 2016 Dec 1, Am J Physiol Lung Cell Mol Physiol
    Read more
  • Increased Meningeal T and Plasma Cell Infiltration is Associated with Early Subpial Cortical Demyelination in Common Marmosets with Experimental Autoimmune Encephalomyelitis. Kramann N et al., 2015 May, Brain Pathol
    Read more
  • CD14(+)S100A9(+) monocytic myeloid-derived suppressor cells and their clinical relevance in non-small cell lung cancer. Feng PH et al., 2012 Nov 15, Am J Respir Crit Care Med
    Read more

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Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9677) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13 kDa. The expected band size for S100A9 is at 13 kDa.
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