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MAD1L1 Antibody (YA2449)

HY-P82704
MedChem Express
TargetMAD1L1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    MAD1L1 Antibody (YA2449)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8379
  • Target name
    MAD1L1
  • Target description
    mitotic arrest deficient 1 like 1
  • Target synonyms
    MAD1; MAD1 mitotic arrest deficient like 1; MAD1-like protein 1; mitotic arrest deficient 1-like protein 1; mitotic checkpoint MAD1 protein homolog; mitotic spindle assembly checkpoint protein MAD1; mitotic-arrest deficient 1, yeast, homolog-like 1; PIG9; tax-binding protein 181; TP53I9; tumor protein p53 inducible protein 9; TXBP181
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    MAD1L1 Antibody (YA2449) is a non-conjugated IgG antibody, targeting MAD1L1, with a predicted molecular weight of 83 kDa (observed band size: 80 kDa). MAD1L1 Antibody (YA2449) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAD1 using anti-MAD1 antibody (PB9262). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. Lane 5: human HL-60 whole cell lysates. Lane 6: human THP-1 whole cell lysates. Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody (Catalog # PB9262) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.
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