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Western Blot analysis of Colo205 cells using MADD Polyclonal Antibody
Western Blot analysis of Colo205 cells using MADD Polyclonal Antibody
Western Blot analysis of Colo205 cells using MADD Polyclonal Antibody

MADD Antibody

CSB-PA003185
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMADD
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Overview

  • Supplier
    Cusabio
  • Product Name
    MADD Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8567
  • Target name
    MADD
  • Target description
    MAP kinase activating death domain
  • Target synonyms
    DEEAH, DENN, IG20, NEDDISH, RAB3GEP, RabGEF, MAP kinase-activating death domain protein, Insuloma-Glucagonoma protein 20, Rab3 GDP/GTP exchange factor, differentially expressed in normal and neoplastic cells, insulinoma glucagonoma clone 20, rab3 GDP/GTP exchange protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXG6
  • Protein Name
    MAP kinase-activating death domain protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MADD using anti-MADD antibody (A02030-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADD antigen affinity purified polyclonal antibody (Catalog # A02030-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADD at approximately 240 kDa. The expected band size for MADD is at 183 kDa.
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