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Western Blot analysis of COLO205 cells using Emp Polyclonal Antibody
Western Blot analysis of COLO205 cells using Emp Polyclonal Antibody
Western Blot analysis of COLO205 cells using Emp Polyclonal Antibody

MAEA Antibody

CSB-PA002337
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAEA
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAEA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10296
  • Target name
    MAEA
  • Target description
    macrophage erythroblast attacher, E3 ubiquitin ligase
  • Target synonyms
    cell proliferation-inducing gene 5 protein; E3 ubiquitin-protein transferase MAEA; EMLP; EMP; erythroblast macrophage protein; GID complex subunit 9, FYV10 homolog; GID9; HLC-10; human lung cancer oncogene 10 protein; lung cancer-related protein 10; P44EMLP; PIG5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L5Y9
  • Protein Name
    Macrophage erythroblast attacher
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAEA using anti-MAEA antibody (A08766-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAEA antigen affinity purified polyclonal antibody (Catalog # A08766-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAEA at approximately 45 kDa. The expected band size for MAEA is at 45 kDa.
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