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MAEA Polyclonal Antibody

CAC14737
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAEA
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Overview

  • Supplier
    Biomatik
  • Product Name
    MAEA Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10296
  • Target name
    MAEA
  • Target description
    macrophage erythroblast attacher, E3 ubiquitin ligase
  • Target synonyms
    EMLP, EMP, GID9, HLC-10, P44EMLP, PIG5, E3 ubiquitin-protein transferase MAEA, GID complex subunit 9, FYV10 homolog, cell proliferation-inducing gene 5 protein, erythroblast macrophage protein, human lung cancer oncogene 10 protein, lung cancer-related protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L5Y9
  • Protein Name
    Macrophage erythroblast attacher
  • Scientific Description
    The MAEA Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAEA using anti-MAEA antibody (A08766-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAEA antigen affinity purified polyclonal antibody (Catalog # A08766-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAEA at approximately 45 kDa. The expected band size for MAEA is at 45 kDa.
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