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WB analysis of human brain tissue lysate using GTX04828 MAFA antibody. The lane on the left was treated with blocking peptide.
WB analysis of human brain tissue lysate using GTX04828 MAFA antibody. The lane on the left was treated with blocking peptide.
WB analysis of human brain tissue lysate using GTX04828 MAFA antibody. The lane on the left was treated with blocking peptide.

MAFA antibody

GTX04828
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMAFA
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAFA antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID389692
  • Target name
    MAFA
  • Target description
    MAF bZIP transcription factor A
  • Target synonyms
    INSDM, RIPE3b1, hMafA, transcription factor MafA, RIPE3b1 activator, RIPE3b1 factor, pancreatic beta-cell-specific transcriptional activator, transcription factor RIPE3b1, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NHW3
  • Protein Name
    Transcription factor MafA
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of MAFA using anti-MAFA antibody (A05251-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody (Catalog # A05251-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFA at approximately 50KD. The expected band size for MAFA is at 37KD.
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