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MAFA Antibody

LS-C832669
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMAFA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAFA Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID389692
  • Target name
    MAFA
  • Target description
    MAF bZIP transcription factor A
  • Target synonyms
    INSDM, RIPE3b1, hMafA, transcription factor MafA, RIPE3b1 activator, RIPE3b1 factor, pancreatic beta-cell-specific transcriptional activator, transcription factor RIPE3b1, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAFA using anti-MAFA antibody (A05251-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody (Catalog # A05251-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFA at approximately 50KD. The expected band size for MAFA is at 37KD.
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