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MAGE-D4/MAGE-D4B (H-9)

SC-390918
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMAGED4B
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    MAGE-D4/MAGE-D4B (H-9)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID81557
  • Target name
    MAGED4B
  • Target description
    MAGE family member D4B
  • Target synonyms
    melanoma-associated antigen D4, MAGE-D4 antigen, MAGE-E1 antigen, melanoma antigen family D, 4B, melanoma antigen family D4B
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAGED4 using anti-MAGED4 antibody (A16674-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGED4 antigen affinity purified polyclonal antibody (Catalog # A16674-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAGED4 at approximately 100 kDa. The expected band size for MAGED4 is at 100 kDa.
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