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ICC/IF analysis of mixed neuron/glia cell cultures using GTX30663 MAP2 antibody. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative.
ICC/IF analysis of mixed neuron/glia cell cultures using GTX30663 MAP2 antibody. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative.
ICC/IF analysis of mixed neuron/glia cell cultures using GTX30663 MAP2 antibody. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative.

MAP2 antibody

GTX30663
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:10000 - 1:20000. ICC/IF: 1:5000 - 1:10000. IHC-Fr: 1:1000 - 1:5000. IHC: 1:1000 - 1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Chicken
  • Isotype
    IgY
  • Scientific Description
    Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons.
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Netrin-1 promotes excitatory synaptogenesis between cortical neurons by initiating synapse assembly. Goldman JS et al., 2013 Oct 30, J Neurosci
    Read this paper
  • Molecular characterization of the mouse superior lateral parabrachial nucleus through expression of the transcription factor Runx1. Zagami CJ et al., 2010 Nov 11, PLoS One
    Read this paper

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