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ICC/IF analysis of neuron-glial cell cultures from E20 rat using GTX82661 MAP2 antibody. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Red : MAP2 Green : Tau Blue : DAPI Dilution : 1:10,000
ICC/IF analysis of neuron-glial cell cultures from E20 rat using GTX82661 MAP2 antibody. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Red : MAP2 Green : Tau Blue : DAPI Dilution : 1:10,000
ICC/IF analysis of neuron-glial cell cultures from E20 rat using GTX82661 MAP2 antibody. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green. Red : MAP2 Green : Tau Blue : DAPI Dilution : 1:10,000

MAP2 antibody

GTX82661
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
DownloadDatasheet
Product group Antibodies
TargetMAP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:20000. ICC/IF: 1:1000-1:20000. IHC: 1:2500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4133
  • Target name
    MAP2
  • Target description
    microtubule associated protein 2
  • Target synonyms
    MAP-2; MAP2A; MAP2B; MAP2C; microtubule-associated protein 2
  • Host
    Chicken
  • Isotype
    IgY
  • Protein IDP11137
  • Protein Name
    Microtubule-associated protein 2
  • Scientific Description
    This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Functional neuronal circuitry and oscillatory dynamics in human brain organoids. Sharf T et al., 2022 Jul 29, Nat Commun
    Read more
  • Bridging Integrator-1 protein loss in Alzheimers disease promotes synaptic tau accumulation and disrupts tau release. Glennon EB et al., 2020, Brain Commun
    Read more

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Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280 kDa. The expected band size for MAP2 is at 200 kDa.
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