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Western Blot analysis of 293T cells using ASK 1 Polyclonal Antibody
Western Blot analysis of 293T cells using ASK 1 Polyclonal Antibody
Western Blot analysis of 293T cells using ASK 1 Polyclonal Antibody

MAP3K5 Antibody

CSB-PA000942
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAP3K5
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP3K5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4217
  • Target name
    MAP3K5
  • Target description
    mitogen-activated protein kinase kinase kinase 5
  • Target synonyms
    ASK1, MAPKKK5, MEKK5, mitogen-activated protein kinase kinase kinase 5, ASK-1, MAP/ERK kinase kinase 5, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, apoptosis signal-regulating kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99683
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ASK1 antibody [C2C3-2], C-term (GTX107921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (A00929-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASK1/MAP3K5 antigen affinity purified polyclonal antibody (Catalog # A00929-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASK1/MAP3K5 at approximately 155 kDa. The expected band size for ASK1/MAP3K5 is at 155 kDa.
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