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ProductsBack/MAP3K5 / ASK1 Antibody (Ser966)

MAP3K5 / ASK1 Antibody (Ser966)

LS-C772774
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAP3K5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MAP3K5 / ASK1 Antibody (Ser966)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:20000), IHC-P, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4217
  • Target name
    MAP3K5
  • Target description
    mitogen-activated protein kinase kinase kinase 5
  • Target synonyms
    apoptosis signal-regulating kinase 1; ASK1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (A00929-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASK1/MAP3K5 antigen affinity purified polyclonal antibody (Catalog # A00929-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASK1/MAP3K5 at approximately 155 kDa. The expected band size for ASK1/MAP3K5 is at 155 kDa.
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