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Western Blot analysis of 293 cells using COT Polyclonal Antibody
Western Blot analysis of 293 cells using COT Polyclonal Antibody
Western Blot analysis of 293 cells using COT Polyclonal Antibody

MAP3K8 Antibody

CSB-PA001760
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP3K8
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP3K8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1326
  • Target name
    MAP3K8
  • Target description
    mitogen-activated protein kinase kinase kinase 8
  • Target synonyms
    AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT, mitogen-activated protein kinase kinase kinase 8, Ewing sarcoma transformant, augmented in rheumatoid arthritis 2, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41279
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K8 antigen affinity purified polyclonal antibody (Catalog # PB10007) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K8 at approximately 53KD. The expected band size for MAP3K8 is at 53KD.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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