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ProductsBack/MAP4K3 (9T1) Monoclonal Antibody
Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HepG2 Cell lysates; Lane 2: MCF-7 Cell lysates; probed with MAP4K3 (219CT8.3.1) Monoclonal Antibody, unconjugated (bsm-51067M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

MAP4K3 (9T1) Monoclonal Antibody

BSM-51067M
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMAP4K3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    MAP4K3 (9T1) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9T1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID8491
  • Target name
    MAP4K3
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 3
  • Target synonyms
    germinal center kinase-like kinase; germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1
  • Host
    Mouse
  • Isotype
    IgM
  • Protein IDQ8IVH8
  • Protein Name
    Mitogen-activated protein kinase kinase kinase kinase 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP4K3 using anti-MAP4K3 antibody (A07188-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K3 antigen affinity purified polyclonal antibody (Catalog # A07188-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K3 at approximately 100 kDa. The expected band size for MAP4K3 is at 101 kDa.
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