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Western Blot analysis of K562 cells using GLK Polyclonal Antibody
Western Blot analysis of K562 cells using GLK Polyclonal Antibody
Western Blot analysis of K562 cells using GLK Polyclonal Antibody

MAP4K3 Antibody

CSB-PA002697
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP4K3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP4K3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8491
  • Target name
    MAP4K3
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 3
  • Target synonyms
    GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1, mitogen-activated protein kinase kinase kinase kinase 3, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, germinal center kinase-like kinase, germinal center kinase-related protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IVH8
  • Protein Name
    Mitogen-activated protein kinase kinase kinase kinase 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MAP4K3 using anti-MAP4K3 antibody (A07188-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K3 antigen affinity purified polyclonal antibody (Catalog # A07188-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K3 at approximately 100 kDa. The expected band size for MAP4K3 is at 101 kDa.
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