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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA013440DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA013440DSR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA013440DSR1HU at dilution of 1:100

MAP4K5 Antibody

CSB-PA013440DSR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMAP4K5
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Overview

  • Supplier
    Cusabio
  • Product Name
    MAP4K5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11183
  • Target name
    MAP4K5
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 5
  • Target synonyms
    GCKR, KHS, KHS1, MAPKKKK5, mitogen-activated protein kinase kinase kinase kinase 5, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5, MEKKK 5, germinal center kinase-related, kinase homologous to SPS1/STE20
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4K4
  • Protein Name
    Mitogen-activated protein kinase kinase kinase kinase 5
  • Scientific Description
    May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Mouse Cerebellum lysates probed with MAP4K5 Polyclonal Antibody, Unconjugated (bs-13654R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
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