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Anti-MAP4K5 Antibody

101-10951
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMAP4K5
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MAP4K5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11183
  • Target name
    MAP4K5
  • Target description
    mitogen-activated protein kinase kinase kinase kinase 5
  • Target synonyms
    GCKR, KHS, KHS1, MAPKKKK5, mitogen-activated protein kinase kinase kinase kinase 5, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5, MEKKK 5, germinal center kinase-related, kinase homologous to SPS1/STE20
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human MAP4K5 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
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