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ProductsBack/MAPK11 Mouse Monoclonal Antibody [Clone ID: OTI1C2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK11 (Cat# RC206583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK11 (Cat# TA501085). Positive lysates [LY400971] (100ug) and [LC400971] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK11 (Cat# RC206583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK11 (Cat# TA501085). Positive lysates [LY400971] (100ug) and [LC400971] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK11 (Cat# RC206583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK11 (Cat# TA501085). Positive lysates [LY400971] (100ug) and [LC400971] (20ug) can be purchased separately from OriGene.

MAPK11 Mouse Monoclonal Antibody [Clone ID: OTI1C2]

CF501085
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK11
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MAPK11 Mouse Monoclonal Antibody [Clone ID: OTI1C2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1C2
  • Gene ID5600
  • Target name
    MAPK11
  • Target description
    mitogen-activated protein kinase 11
  • Target synonyms
    P38B, P38BETA2, PRKM11, SAPK2, SAPK2B, p38-2, p38Beta, mitogen-activated protein kinase 11, MAP kinase 11, MAP kinase p38 beta, mitogen-activated protein kinase p38 beta, mitogen-activated protein kinase p38-2, stress-activated protein kinase-2, stress-activated protein kinase-2b
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15759
  • Protein Name
    Mitogen-activated protein kinase 11
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MAPK11 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MAPK11 using anti-MAPK11 antibody (A03738-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SH-5Y5Y whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: rat lung tissue lysates, Lane 9: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK11 antigen affinity purified polyclonal antibody (Catalog # A03738-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK11 at approximately 45KD. The expected band size for MAPK11 is at 45KD.
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