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ProductsBack/Goat anti-ERK3 / MAPK6 Antibody

Goat anti-ERK3 / MAPK6 Antibody

ORB107650
Biorbyt
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAPK6
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Mapk6 antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA: 1:128000, WB: 0.5-1 μg/ml, IHC-P: 10 μg/ml ELISA, IHC, IP, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5597
  • Target name
    MAPK6
  • Target description
    mitogen-activated protein kinase 6
  • Target synonyms
    ERK3; ERK-3; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; HsT17250; MAP kinase 6; MAPK 6; mitogen-activated protein kinase 6; p97MAPK; p97-MAPK; PRKM6; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to Mapk6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAPK6 using anti-MAPK6 antibody (PB9666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug, Lane 4: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK6 antigen affinity purified polyclonal antibody (Catalog # PB9666) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK6 at approximately 100 kDa. The expected band size for MAPK6 is at 68 kDa.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with MAPK6/ERK3 Polyclonal Antibody, Unconjugated (bs-12405R)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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