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ProductsBack/MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK6 (Cat# RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6(Cat# TA500077). Positive lysates [LY400969] (100ug) and [LC400969] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK6 (Cat# RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6(Cat# TA500077). Positive lysates [LY400969] (100ug) and [LC400969] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK6 (Cat# RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6(Cat# TA500077). Positive lysates [LY400969] (100ug) and [LC400969] (20ug) can be purchased separately from OriGene.

MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]

CF500077
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPK6
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5E1
  • Gene ID5597
  • Target name
    MAPK6
  • Target description
    mitogen-activated protein kinase 6
  • Target synonyms
    ERK3, HsT17250, PRKM6, p97MAPK, mitogen-activated protein kinase 6, ERK-3, MAP kinase 6, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activated 5, protein kinase, mitogen-activated 6
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ16659
  • Protein Name
    Mitogen-activated protein kinase 6
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MAPK6 using anti-MAPK6 antibody (PB9666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug, Lane 4: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK6 antigen affinity purified polyclonal antibody (Catalog # PB9666) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK6 at approximately 100 kDa. The expected band size for MAPK6 is at 68 kDa.
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