ProductsBack/MAPK8/MAPK9/MAPK10 Antibody

MAPK8/MAPK9/MAPK10 Antibody

MBS7122129

Product group Antibodies

Overview

Supplier
MyBioSource
Product Name
MAPK8/MAPK9/MAPK10 Antibody
Delivery Days Customer
16
Certification
Research Use Only
Isotype
IgG
Storage Instruction
-20°C or -80°C
UNSPSC
12352203

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Figure 1. Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.

Product group Antibodies

References

Anti-JNK1/2/3 Rabbit Monoclonal AntibodyM02608-1

ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry

ReactivityHuman, Mouse, Rat

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Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Product group Antibodies

References

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$Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.$

Product group Antibodies

References

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