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ProductsBack/MAPRE3 Recombinant Antibody, AbBy Fluor-405 Conjugated

MAPRE3 Recombinant Antibody, AbBy Fluor-405 Conjugated

BSM-61869R-BF405
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAPRE3
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Overview

  • Supplier
    Bioss
  • Product Name
    MAPRE3 Recombinant Antibody, AbBy Fluor-405 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB(1:300-5000), IF(), FCM(1ug/Test)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID22924
  • Target name
    MAPRE3
  • Target description
    microtubule associated protein RP/EB family member 3
  • Target synonyms
    EB3, EBF3, EBF3-S, RP3, microtubule-associated protein RP/EB family member 3, APC binding protein, EB1 protein family member 3, end-binding protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPY8
  • Protein Name
    Microtubule-associated protein RP/EB family member 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of EB3/MAPRE3 using anti-EB3/MAPRE3 antibody (A05782-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EB3/MAPRE3 antigen affinity purified polyclonal antibody (Catalog # A05782-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EB3/MAPRE3 at approximately 35 kDa. The expected band size for EB3/MAPRE3 is at 32 kDa.
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