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Western blot All lanes: MARCO antibody at 1.97microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 44 kDa Observed band size: 53 kDa
Western blot All lanes: MARCO antibody at 1.97microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 44 kDa Observed band size: 53 kDa
Western blot All lanes: MARCO antibody at 1.97microg/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 44 kDa Observed band size: 53 kDa

MARCO Antibody

CSB-PA880072ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMARCO
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Overview

  • Supplier
    Cusabio
  • Product Name
    MARCO Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8685
  • Target name
    MARCO
  • Target description
    macrophage receptor with collagenous structure
  • Target synonyms
    SCARA2, SR-A6, macrophage receptor MARCO, scavenger receptor class A, member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UEW3
  • Protein Name
    Macrophage receptor MARCO
  • Scientific Description
    Pattern recognition receptor (PRR). Binds Gram-positive and Gram-negative bacteria.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MARCO using anti-MARCO antibody (A00391-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCO antigen affinity purified polyclonal antibody (A00391-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARCO at approximately 53 kDa. The expected band size for MARCO is at 53 kDa.
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