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ELISA analysis of antigen using GTX60648 Mark3 antibody [2G12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60648 Mark3 antibody [2G12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60648 Mark3 antibody [2G12]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng

Mark3 antibody [2G12]

GTX60648
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMARK3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Mark3 antibody [2G12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2G12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4140
  • Target name
    MARK3
  • Target description
    microtubule affinity regulating kinase 3
  • Target synonyms
    CTAK1, KP78, PAR1A, Par-1a, VIPB, MAP/microtubule affinity-regulating kinase 3, C-TAK1, ELKL motif kinase 2, EMK-2, cdc25C-associated protein kinase 1, protein kinase STK10, ser/Thr protein kinase PAR-1, serine/threonine-protein kinase p78
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP27448
  • Protein Name
    MAP/microtubule affinity-regulating kinase 3
  • Scientific Description
    The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MARK3 using anti-MARK3 antibody (A05355-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse EL-4 whole cell lysates, Lane 5: mouse 4T1 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (Catalog # A05355-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.
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