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Western blot analysis of extracts from HeLa cells and 293 cells, using MARK3 antibody.
Western blot analysis of extracts from HeLa cells and 293 cells, using MARK3 antibody.
Western blot analysis of extracts from HeLa cells and 293 cells, using MARK3 antibody.

MARK3 Antibody

CSB-PA052531
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMARK3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MARK3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4140
  • Target name
    MARK3
  • Target description
    microtubule affinity regulating kinase 3
  • Target synonyms
    cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 2; EMK-2; KP78; MAP/microtubule affinity-regulating kinase 3; PAR1A; Par-1a; protein kinase STK10; ser/Thr protein kinase PAR-1; serine/threonine-protein kinase p78; VIPB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27448
  • Protein Name
    MAP/microtubule affinity-regulating kinase 3
  • Scientific Description
    Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on Ser-216. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Peng C.Y., Cell Growth Differ. 9:197-208(1998). Sun T.-Q., Nat. Cell Biol. 3:628-636(2001). Wissing J., Mol. Cell. Proteomics 6:537-547(2007)
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MARK3 using anti-MARK3 antibody (A05355-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse EL-4 whole cell lysates, Lane 5: mouse 4T1 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (Catalog # A05355-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.
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Lane 1: Rat Brain; Lane 2: A431 Cells; Lane 3: 293 Cells; Probed with MARK3 (2F7) Monoclonal Antibody (bsm-54462R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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