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MASP2 / MASP-2 Antibody (FITC)

LS-C417866
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetMASP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MASP2 / MASP-2 Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ICC (1:100 - 1:500), WB (1:100 - 1:400) Applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID10747
  • Target name
    MASP2
  • Target description
    MBL associated serine protease 2
  • Target synonyms
    mannan binding lectin serine peptidase 2; mannan-binding lectin serine peptidase 1 pseudogene 1; mannan-binding lectin serine protease 1 pseudogene 1; mannan-binding lectin serine protease 2; mannose-binding lectin associated protein 19; mannose-binding lectin-associated serine protease 2; mannose-binding protein-associated serine protease 2; MAP19; MAP-2; MASP1P1; MASP-2; MBL-associated plasma protein of 19 kD; small MBL-associated protein; sMAP
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody (A02323-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASP2 antigen affinity purified polyclonal antibody (Catalog # A02323-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASP2 at approximately 76KD. The expected band size for MASP2 is at 76KD.
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