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IHC analysis of MRE11 using anti-MRE11 antibody (A00731-2).
Product group Antibodies
Boster Bio
Anti-MRE11/MRE11 Antibody Picoband(r)A00731-2
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMRE11
Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.
Product group Antibodies
Boster Bio
Anti-MIP-3 Alpha/CCL20 Antibody Picoband(r)A00748-2
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetCcl20
Western blot analysis of DDX3 using anti-DDX3 antibody (A00751-1).
Product group Antibodies
Boster Bio
Anti-DDX3/DDX3X Antibody Picoband(r)A00751-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetDDX3X
Western blot analysis of CCR4 using anti-CCR4 antibody (A00755).
Product group Antibodies
Boster Bio
Anti-CCR4 Antibody Picoband(r)A00755
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCCR4
Western blot analysis of PARK7 / DJ1 using anti-PARK7 / DJ1 antibody (A00757-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARK7 / DJ1 antigen affinity purified polyclonal antibody (Catalog # A00757-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARK7 / DJ1 at approximately 22KD. The expected band size for PARK7 / DJ1 is at 20KD.
Product group Antibodies
Boster Bio
Anti-PARK7/DJ1 Antibody Picoband(r)A00757-2
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPark7
Flow Cytometry analysis of U20S cells using anti-SOX10 antibody (A00758-1). Overlay histogram showing U20S cells stained with A00758-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOX10 Antibody (A00758-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-SOX10 Antibody Picoband(r)A00758-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSOX10
Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1).
Product group Antibodies
Boster Bio
Anti-LOX-1/OLR1 Antibody Picoband(r)A00760-1
ApplicationsWestern Blot
ReactivityHuman
TargetOLR1
IHC analysis of PAH using anti-PAH antibody (A00761-1). PAH was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PAH Antibody (A00761-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-PAH Antibody Picoband(r)A00761-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAH
Western blot analysis of AMH using anti-AMH antibody (A00763).
Product group Antibodies
Boster Bio
Anti-AMH Antibody Picoband(r)A00763
ApplicationsWestern Blot
ReactivityHuman
TargetAMH
Western blot analysis of Ogg1 using anti-Ogg1 antibody (A00768-1).
Product group Antibodies
Boster Bio
Anti-Ogg1 Antibody Picoband(r)A00768-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetOGG1
Western blot analysis of TLS / FUS using anti-TLS / FUS antibody (A00771-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLS / FUS antigen affinity purified polyclonal antibody (Catalog # A00771-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLS / FUS at approximately 75KD. The expected band size for TLS / FUS is at 53KD.
Product group Antibodies
Boster Bio
Anti-TLS/FUS Antibody Picoband(r)A00771-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetFUS
Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1).
Product group Antibodies
Boster Bio
Anti-Estrogen Receptor beta/ESR2 Antibody Picoband(r)A00786-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetESR2
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