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Western blot analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody (M01239-1).
Product group Antibodies
Boster Bio
Anti-HAUSP/USP7 Antibody Picoband(r) (monoclonal, 5E2)M01239-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetUSP7
Western blot analysis of USP7 expression in Ramos cell lysate using anti-USP7 antibody (M01239). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP7 antigen affinity purified polyclonal antibody (Catalog # M01239) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for USP7.
Product group Antibodies
Boster Bio
Anti-HAUSP / USP7 Rabbit Monoclonal AntibodyM01239
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetUSP7
Western blot analysis of CD62P expression in HUVEC cell lysate.
Product group Antibodies
Boster Bio
Anti-CD62P SELP Rabbit Monoclonal AntibodyM01241
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSELP
Western blot analysis of Bovine Serum Albumin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.
Product group Antibodies
Boster Bio
Anti-Bovine Serum Albumin Rabbit Monoclonal AntibodyM01245
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetALB
Western blot analysis of Occludin expression in HepG2 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Occludin OCLN Rabbit Monoclonal AntibodyM01246
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetOCLN
Western blot analysis of CEBP alpha expression in U937 cell lysate (M00386). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA monoclonal antibody (Catalog # M00386) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBPA
Product group Antibodies
Boster Bio
Anti-CEBP alpha Rabbit Monoclonal AntibodyM00386
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCEBPA
Western blot analysis of ERCC1 expression in HeLa cell lysate (M00388). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 monoclonal antibody (Catalog # M00388) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1
Product group Antibodies
Boster Bio
Anti-ERCC1 Rabbit Monoclonal AntibodyM00388
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetERCC1
Western blot analysis of HNF-4-alpha expression in A549 cell lysate (M00389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A monoclonal antibody (Catalog # M00389) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-HNF-4-alpha Rabbit Monoclonal AntibodyM00389
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetHNF4A
Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Product group Antibodies
Boster Bio
Anti-CCR7 Rabbit Monoclonal AntibodyM00390
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR7
Western blot analysis of Retinoic Acid Receptor alpha expression in MCF-7 cell lysate (M00392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARA monoclonal antibody (Catalog # M00392) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RARA
Product group Antibodies
Boster Bio
Anti-Retinoic Acid Receptor alpha RARA Rabbit Monoclonal AntibodyM00392
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse
TargetRARA
Western blot analysis of Interferon gamma expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Interferon gamma IFNG Rabbit Monoclonal AntibodyM00393-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetIFNG
Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve.
Product group Antibodies
Boster Bio
Anti-Interferon gamma IFNG Antibody (monoclonal, 8E9)M00393-2
ApplicationsELISA
ReactivityRat
TargetIfng
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