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Western blot analysis of DR5 expression in HeLa cell lysate (M00410). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF10B monoclonal antibody (Catalog # M00410) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF10B
Product group Antibodies
Boster Bio
Anti-DR5 Rabbit Monoclonal AntibodyM00410
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetTNFRSF10B
Western blot analysis of MIF expression in (1) THP-1 cell lysate; (2) Mouse brain lysate (M00411). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF monoclonal antibody (Catalog # M00411) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF
Product group Antibodies
Boster Bio
Anti-MIF Rabbit Monoclonal AntibodyM00411
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMIF
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRP8 Antibody.
Product group Antibodies
Boster Bio
Anti-MRP8 S100A8 Rabbit Monoclonal AntibodyM00413-1
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetS100A8
Western blot analysis of MMP13 expression in HeLa cell lysate (M00420). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13 monoclonal antibody (Catalog # M00420) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP13
Product group Antibodies
Boster Bio
Anti-MMP13 Rabbit Monoclonal AntibodyM00420
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMMP13
Western blot analysis of FAP1 expression in U-87 MG cell lysate.
Product group Antibodies
Boster Bio
Anti-FAP1 Rabbit Monoclonal AntibodyM00422
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFAP
Western blot analysis of LCK using anti-LCK antibody in Jurkat cell lysate (M00425). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCK antigen affinity purified polyclonal antibody (Catalog # M00425) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Lck Monoclonal AntibodyM00425
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetLCK
Western blot analysis of TCF7L2 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-TCF7L2 Rabbit Monoclonal AntibodyM00431
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTCF7L2
Western blot analysis of AGTR2 expression in HepG2 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-AGTR2/Angiotensin Ii Type 2 Receptor Rabbit Monoclonal AntibodyM00432
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetAGTR2
Western blot analysis of SIRT2 expression in SKBR-3 cell lysate (M00433-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody (Catalog # M00433-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT2
Product group Antibodies
Boster Bio
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal AntibodyM00433-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetSIRT2
Western blot analysis of SIRT2 expression in SH-SY5Y cell lysate (M00433). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 monoclonal antibody (Catalog # M00433) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT2
Product group Antibodies
Boster Bio
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal AntibodyM00433
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetSIRT2
Immunohistochemical analysis of paraffin-embedded human kidney, using Lamin A/C Antibody.
Product group Antibodies
Boster Bio
Anti-Lamin A/C LMNA Rabbit Monoclonal AntibodyM00438
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetLMNA
Western blot analysis of Calreticulin expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-Daxx Rabbit Monoclonal AntibodyM00439
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDAXX
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