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Western blot analysis of SMC1 expression in (1) HeLa cell lysate; (2) Jurkat cell lysate (M02148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A monoclonal antibody (Catalog # M02148) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A
Product group Antibodies
Boster Bio
Anti-SMC1 SMC1A Rabbit Monoclonal AntibodyM02148
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMC1A
IImmunohistochemical analysis of paraffin-embedded human thyroid, using DUSP6 Antibody.
Product group Antibodies
Boster Bio
Anti-DUSP6/Mkp 3 Rabbit Monoclonal AntibodyM02157
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDUSP6
Western blot analysis of Cdc37 expression in (1) Jurkat cell lysate; (2) SW480 cell lysate.
Product group Antibodies
Boster Bio
Anti-Cdc37 Rabbit Monoclonal AntibodyM02169
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDC37
Western blot analysis of SNRPN using anti-SNRPN antibody (M02173).
Product group Antibodies
Boster Bio
Anti-SNRPN Antibody Picoband(r) (monoclonal, 6F12)M02173
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetSNRPN
Western blot analysis of HDAC9 expression in K562 cell lysate (M02177). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC9 monoclonal antibody (Catalog # M02177) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC9
Product group Antibodies
Boster Bio
Anti-HDAC9 Rabbit Monoclonal AntibodyM02177
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetHDAC9
Western blot analysis of NUP153 expression in K562 cell lysate using anti-NUP153 antibody (M02183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP153 antigen affinity purified polyclonal antibody (Catalog # M02183) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NUP153.
Product group Antibodies
Boster Bio
Anti-NUP153 Monoclonal AntibodyM02183
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetNUP153
Western blot analysis of Glycophorin A (CD235a) expression in human fetal liver lysate.
Product group Antibodies
Boster Bio
Anti-Glycophorin A (CD235a) GYPA Monoclonal AntibodyM02184-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetGYPA
Immunohistochemical analysis of paraffin-embedded human kidney, using ARRB1 Antibody(M02185) ARRB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARRB1 Antibody (M02185)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-ARRB1/Beta Arrestin 1 Rabbit Monoclonal AntibodyM02185
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetARRB1
Western blot analysis of PSMB8 expression in U937 cell lysate using anti-PSMB8 antibody (M02188-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB8 antigen affinity purified polyclonal antibody (Catalog # M02188-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PSMB8.
Product group Antibodies
Boster Bio
Anti-PSMB8 / LMP7 Monoclonal AntibodyM02188-2
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPSMB8
Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
Product group Antibodies
Boster Bio
Anti-Nesprin 1 Rabbit Monoclonal AntibodyM02192
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSYNE1
Western blot analysis of SCGB1A1 using anti-SCGB1A1 antibody (M02201) in A549 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCGB1A1 antigen affinity purified polyclonal antibody (Catalog # M02201) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Uteroglobin SCGB1A1 Monoclonal AntibodyM02201
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetSCGB1A1
Western blot analysis of CRX using anti-CRX antibody on Y79 cell lysate(M02202). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRX antigen affinity purified polyclonal antibody (Catalog # M02202) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-CRX Monoclonal AntibodyM02202
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCRX
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