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Product group Antibodies
Boster Bio
Anti-ADAMTS1 Picoband AntibodyA03009-1
Western blot analysis of IL15RA using anti-IL15RA antibody (A03016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL15RA antigen affinity purified polyclonal antibody (Catalog # A03016-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL15RA at approximately 28KD. The expected band size for IL15RA is at 28KD.
Product group Antibodies
Boster Bio
Anti-IL15RA AntibodyA03016-1
Western blot analysis of FBXL11 using anti-FBXL11 antibody (A03027-1).
Product group Antibodies
Boster Bio
Anti-FBXL11/KDM2A AntibodyA03027-1
IHC analysis of ALDH1A3 using anti-ALDH1A3 antibody (A03030).
Product group Antibodies
Boster Bio
Anti-ALDH1A3 AntibodyA03030
Western blot analysis of COL17A1 using anti-COL17A1 antibody (A03031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 antigen affinity purified polyclonal antibody (Catalog # A03031-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL17A1 at approximately 125KD. The expected band size for COL17A1 is at 150KD.
Product group Antibodies
Boster Bio
Anti-Collagen XVII/COL17A1 AntibodyA03031-1
IHC analysis of Calbindin using anti-Calbindin antibody (A03047). Calbindin was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calbindin Antibody (A03047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Calbindin/CALB1 AntibodyA03047
Western blot analysis of E2F3 using anti-E2F3 antibody (A03068).
Product group Antibodies
Boster Bio
Anti-E2F3 Picoband AntibodyA03068
Western blot analysis of TRAF5 using anti-TRAF5 antibody (A03076-1).
Product group Antibodies
Boster Bio
Anti-TRAF5 AntibodyA03076-1
IHC analysis of Aquaporin 5 using anti-Aquaporin 5 antibody (A03085). Aquaporin 5 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Aquaporin 5 Antibody (A03085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Aquaporin 5/AQP5 AntibodyA03085
Western blot analysis of NOX5 using anti-NOX5 antibody (A03090).
Product group Antibodies
Boster Bio
Anti-NOX5 AntibodyA03090
Western blot analysis of XPB using anti-XPB antibody (A03103-1).
Product group Antibodies
Boster Bio
Anti-XPB/ERCC3 AntibodyA03103-1
Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 28KD. The expected band size for TNFRSF18 is at 26KD.
Product group Antibodies
Boster Bio
Anti-TNFRSF18 AntibodyA03125-1
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