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Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNF Receptor I/TNFRSF1A AntibodyA00294-3
Western blot analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 60KD. The expected band size for TNFRSF1A is at 50KD.
Product group Antibodies
Boster Bio
Anti-TNFRSF1A AntibodyA00294
IHC analysis of ErbB 4 using anti-ErbB 4 antibody (A00296).
Product group Antibodies
Boster Bio
Anti-ErbB 4/ERBB4 AntibodyA00296
Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-2).
Product group Antibodies
Boster Bio
Anti-BAFF Receptor/Tnfrsf13c AntibodyA02865-2
Western blot analysis of PLK2 using anti-PLK2 antibody (A02881).
Product group Antibodies
Boster Bio
Anti-PLK2 AntibodyA02881
Western blot analysis of ADAMTS4 using anti-ADAMTS4 antibody (A02899).
Product group Antibodies
Boster Bio
Anti-ADAMTS4 Picoband AntibodyA02899
Western blot analysis of Exportin-5/XPO5 using anti-Exportin-5/XPO5 antibody (A02900).
Product group Antibodies
Boster Bio
Anti-Exportin-5 AntibodyA02900
Western blot analysis of Integrin alpha 3 using anti-Integrin alpha 3 antibody (A02902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 3 antigen affinity purified polyclonal antibody (Catalog # A02902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 3 at approximately 130KD. The expected band size for Integrin alpha 3 is at 116KD.
Product group Antibodies
Boster Bio
Anti-Integrin alpha 3/ITGA3 AntibodyA02902
Western blot analysis of RAB6A using anti-RAB6A antibody (A02911-1).
Product group Antibodies
Boster Bio
Anti-RAB6A AntibodyA02911-1
Western blot analysis of TUB 1 using anti-TUB 1 antibody (A02917-1).
Product group Antibodies
Boster Bio
Anti-TUB 1 Picoband AntibodyA02917-1
IHC analysis of Anterior Gradient 2 using anti-Anterior Gradient 2 antibody (A02922-2).
Product group Antibodies
Boster Bio
Anti-Anterior Gradient 2 Picoband AntibodyA02922-2
Western blot analysis of HSD17B4 using anti-HSD17B4 antibody (A02923).
Product group Antibodies
Boster Bio
Anti-HSD17B4 AntibodyA02923
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