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Western blot analysis of LCP1 using anti-LCP1 antibody (A03361). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (Catalog # A03361) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70KD. The expected band size for LCP1 is at 70KD.
Product group Antibodies
Boster Bio
Anti-LCP1/plastin-2 AntibodyA03361
IHC analysis of Vitamin D Binding protein using anti-Vitamin D Binding protein antibody (A03364-1). Vitamin D Binding protein was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Vitamin D Binding protein Antibody (A03364-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Vitamin D Binding protein AntibodyA03364-1
Western blot analysis of Gc using anti-Gc antibody (A03364-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse liver tissue lysate, Lane 4: mouse liver tissue lysate, Lane 5: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gc antigen affinity purified polyclonal antibody (Catalog # A03364-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gc at approximately 53KD. The expected band size for Gc is at 53KD.
Product group Antibodies
Boster Bio
Anti-Vitamin D Binding protein/Gc AntibodyA03364-2
Western blot analysis of IL12RB1 using anti-IL12RB1 antibody (A03401-1).
Product group Antibodies
Boster Bio
Anti-IL12RB1 Picoband AntibodyA03401-1
Western blot analysis of TSG6 using anti-TSG6 antibody (A03433-1).
Product group Antibodies
Boster Bio
Anti-TSG6 AntibodyA03433-1
Western blot analysis of SFTPB using anti-SFTPB antibody (A03441-1).
Product group Antibodies
Boster Bio
Anti-Prosurfactant Protein B/SFTPB AntibodyA03441-1
Western blot analysis of SLC31A1/CTR1 using anti-SLC31A1/CTR1 antibody (A03447-1).
Product group Antibodies
Boster Bio
Anti-SLC31A1/CTR1 AntibodyA03447-1
IHC analysis of Calpain 2 using anti-Calpain 2 antibody (A03492). Calpain 2 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain 2 Antibody (A03492) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Calpain 2 AntibodyA03492
Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1).
Product group Antibodies
Boster Bio
Anti-LMO2 AntibodyA03502-1
Western blot analysis of LIGHT using anti-LIGHT antibody (A03516-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse cardiac muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT antigen affinity purified polyclonal antibody (Catalog # A03516-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT at approximately 26KD. The expected band size for LIGHT is at 26KD.
Product group Antibodies
Boster Bio
Anti-LIGHT AntibodyA03516-1
Western blot analysis of Laminin using anti-Laminin antibody (A03522-1).
Product group Antibodies
Boster Bio
Anti-Laminin AntibodyA03522-1
IHC analysis of Laminin using anti-Laminin antibody (A03522).
Product group Antibodies
Boster Bio
Anti-Laminin AntibodyA03522
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