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IHC analysis of Cytochrome C using anti-Cytochrome C antibody (A03529).
Product group Antibodies
Boster Bio
Anti-Cytochrome C AntibodyA03529
IHC analysis of DVL1 using anti-DVL1 antibody (A03533-1).
Product group Antibodies
Boster Bio
Anti-DVL1 AntibodyA03533-1
Western blot analysis of ZP3 using anti-ZP3 antibody (A03543-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP3 antigen affinity purified polyclonal antibody (Catalog # A03543-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP3 at approximately 47KD. The expected band size for ZP3 is at 47KD.
Product group Antibodies
Boster Bio
Anti-ZP3 AntibodyA03543-1
IHC analysis of MED15 using anti-MED15 antibody (A03568-1). MED15 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (A03568-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-MED15 AntibodyA03568-1
Western blot analysis of PLCG 2/PLCG2 using anti-PLCG 2/PLCG2 antibody (A03572-1).
Product group Antibodies
Boster Bio
Anti-PLCG 2/PLCG2 AntibodyA03572-1
Western blot analysis of Dishevelled 3 using anti-Dishevelled 3 antibody (A03577).
Product group Antibodies
Boster Bio
Anti-Dishevelled 3 AntibodyA03577
Western blot analysis of BRMS1 using anti-BRMS1 antibody (A03587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRMS1 antigen affinity purified polyclonal antibody (Catalog # A03587-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRMS1 at approximately 34KD. The expected band size for BRMS1 is at 28KD.
Product group Antibodies
Boster Bio
Anti-BRMS1 AntibodyA03587-1
Product group Antibodies
Boster Bio
Anti-BRMS1 AntibodyA03587-2
Western blot analysis of PCDH15 using anti-PCDH15 antibody (A03591-1).
Product group Antibodies
Boster Bio
Anti-PCDH15 AntibodyA03591-1
Western blot analysis of LMAN1 using anti-LMAN1 antibody (A03628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysate, Lane 2: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMAN1 antigen affinity purified polyclonal antibody (Catalog # A03628-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMAN1 at approximately 58KD. The expected band size for LMAN1 is at 58KD.
Product group Antibodies
Boster Bio
Anti-LMAN1 AntibodyA03628-1
Product group Antibodies
Boster Bio
Anti-Aquaporin 9/AQP9 AntibodyA03638-1
Western blot analysis of MCM5 using anti-MCM5 antibody (A03642).
Product group Antibodies
Boster Bio
Anti-mcm5 AntibodyA03642
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