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Western blot analysis of extracts from HepG2 cells, COLO cells, Jurkat cells, HUVEC cells and COS cells, using PPP4R1L antibody A18497.
Product group Antibodies
Boster Bio
Anti-PPP4R1L AntibodyA18497
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-10UG
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-CARRIER-FREE
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-CY3
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-DYLIGHT594
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-FITC
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-IFLUOR647
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-SHCBP1L Antibody Picoband(r)A15056-2-PE
TargetSHCBP1L
  • SizePrice
Figure 1. Western blot analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tisue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMBP2 antigen affinity purified polyclonal antibody (Catalog # A15057) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMBP2 at approximately 150 kDa. The expected band size for RIMBP2 is at 116 kDa.
Product group Antibodies
Boster Bio
Anti-RIMBP2 Antibody Picoband(r)A15057-HRP
TargetRIMBP2
  • SizePrice
Immunofluorescence analysis of A549 cells, using LDLRAD3 antibody A15061.
Product group Antibodies
Boster Bio
Anti-LDLRAD3 AntibodyA15061
TargetLDLRAD3
  • SizePrice
Figure 1. Immunohistochemistry validation of APOL4 using Anti-Apolipoprotein L4 ApoL4 Antibody (A15071). Immunohistochemical analysis of paraffin-embedded human lung cancer. Antibody was diluted at 1:100 (4°C
Product group Antibodies
Boster Bio
Anti-Apolipoprotein L4 ApoL4 AntibodyA15071
TargetAPOL4
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Neuromedin-S NMS AntibodyA15072-1
TargetNMS
  • SizePrice
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