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IHC analysis of CD11c using anti-CD11c antibody (A00357-1).
Product group Antibodies
Boster Bio
Anti-CD11c/ITGAX AntibodyA00357-1
IHC analysis of Cdk6 using anti-Cdk6 antibody (A00358). Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (A00358) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Cdk6 AntibodyA00358
Western blot analysis of CD47 using anti-CD47 antibody (A00360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD47 antigen affinity purified polyclonal antibody (Catalog # A00360-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD47 at approximately 41KD. The expected band size for CD47 is at 35KD.
Product group Antibodies
Boster Bio
Anti-CD47 AntibodyA00360-1
Western blot analysis of CD47 using anti-CD47 antibody (A00360-2).
Product group Antibodies
Boster Bio
Anti-CD47 AntibodyA00360-2
IHC analysis of ETV6/Tel using anti-ETV6/Tel antibody (A00361-1).
Product group Antibodies
Boster Bio
Anti-ETV6/Tel AntibodyA00361-1
Western blot analysis of iNOS using anti-iNOS antibody (A00368).
Product group Antibodies
Boster Bio
Anti-iNOS Picoband AntibodyA00368
IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody (A00370).
Product group Antibodies
Boster Bio
Anti-Angiopoietin-2 Picoband AntibodyA00370
IHC analysis of ATF4 using anti-ATF4 antibody (A00371-1). ATF4 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ATF4 Antibody (A00371-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-ATF4 AntibodyA00371-1
Western blot analysis of ATF4 using anti-ATF4 antibody (A00371).
Product group Antibodies
Boster Bio
Anti-ATF4 Picoband AntibodyA00371
Product group Antibodies
Boster Bio
Anti-CD24 AntibodyA00373-2
Western blot analysis of EGF using anti-EGF antibody (A00378-1).
Product group Antibodies
Boster Bio
Anti-EGF AntibodyA00378-1
IHC analysis of Survivin using anti-Survivin antibody (A00379). Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Survivin AntibodyA00379
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