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Western blot analysis of APLP2 expression in HeLa cell lysate.
Product group Antibodies
Boster Bio
Anti-APLP2 Rabbit Monoclonal AntibodyM01232
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAPLP2
  • SizePrice
All lanes : Anti-TSG101 at 1:2000 dilution Lane 1: Human brain lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: A431 whole cell lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-TSG101 AntibodyM01233-1
ApplicationsWestern Blot
ReactivityHuman
TargetTSG101
  • SizePrice
All lanes : Anti-TSG101 at 1:2000 dilution Lane 1: Human brain lysate Lane 2: K562 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: A431 whole cell lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-TSG101 AntibodyM01233-1
ApplicationsWestern Blot
ReactivityHuman
TargetTSG101
  • SizePrice
Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (M01233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human COLO320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified monoclonal antibody (Catalog # M01233) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa.
Product group Antibodies
Boster Bio
Anti-TSG101 Rabbit Monoclonal AntibodyM01233-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTSG101
  • SizePrice
Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (M01233). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human COLO320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified monoclonal antibody (Catalog # M01233) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa.
Product group Antibodies
Boster Bio
Anti-TSG101 Rabbit Monoclonal AntibodyM01233
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTSG101
  • SizePrice
Figure 1. Western blot analysis of Dysferlin using anti-Dysferlin antibody (M01234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dysferlin antigen affinity purified monoclonal antibody (Catalog # M01234) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dysferlin at approximately 280 kDa. The expected band size for Dysferlin is at 237 kDa.
Product group Antibodies
Boster Bio
Anti-Dysferlin Rabbit Monoclonal AntibodyM01234-30UL
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetDYSF
  • SizePrice
Figure 1. Western blot analysis of Dysferlin using anti-Dysferlin antibody (M01234). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dysferlin antigen affinity purified monoclonal antibody (Catalog # M01234) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dysferlin at approximately 280 kDa. The expected band size for Dysferlin is at 237 kDa.
Product group Antibodies
Boster Bio
Anti-Dysferlin Rabbit Monoclonal AntibodyM01234
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetDYSF
  • SizePrice
Product group Antibodies
Boster Bio
Anti-HLA-G Purified Azide Free Monoclonal AntibodyM01235-2
ApplicationsFlow Cytometry, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman
TargetHLA-G
  • SizePrice
Western blot analysis of HLA G expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-HLA G Rabbit Monoclonal AntibodyM01235-30UL
ApplicationsWestern Blot
ReactivityHuman
TargetHLA-G
  • SizePrice
Western blot analysis of HLA G expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-HLA G Rabbit Monoclonal AntibodyM01235
ApplicationsWestern Blot
ReactivityHuman
TargetHLA-G
  • SizePrice
Figure 1. Western blot analysis of STUB1 using anti-STUB1 antibody (M01236). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human CACO-2 whole cell lysates. Lane 4: human THP-1 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-STUB1 antigen affinity purified monoclonal antibody (Catalog # M01236) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for STUB1 at approximately 35KD. The expected band size for STUB1 is at 35KD.
Product group Antibodies
Boster Bio
Anti-STUB1 Antibody Picoband(r) (monoclonal, 13I8)M01236-10UG
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetSTUB1
  • SizePrice
All lanes : Anti-STUB1 Antibody (C-term) at 1:4000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: L929 whole cell lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-STUB1 Antibody (C-term)M01236-1
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetSTUB1
  • SizePrice
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