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Western blot analysis of BAD expression in (1) HeLa cell lysate; (2) HeLa cell treated with Calcyculin A lysate using anti-BAD antibody (MP03520). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAD antigen affinity purified polyclonal antibody (Catalog # MP03520) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BAD.
Product group Antibodies
Boster Bio
Anti-Phospho-Bad (S112) Monoclonal AntibodyMP03520
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetBAD
Western blot analysis of Phospho-MSK1 (S376) expression in HEK293 cell lysate treated with EGF.
Product group Antibodies
Boster Bio
Anti-Phospho-MSK1 (S376) RPS6KA5 Monoclonal AntibodyMP04922
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetRPS6KA5
IHC analysis of H3F3A using anti-H3F3A antibody (MP06819). H3F3A was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-H3F3A Antibody (MP06819) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal AntibodyMP06819
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetH3-3A
Immunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H3 (S10) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-Histone H3 (S10) HIST1H3A Rabbit Monoclonal AntibodyMP12477
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH3C1
Western blot analysis of HA-tag fusion protein, using HA tag Antibody
Product group Antibodies
References
Boster Bio
Anti-HA-Tag Rabbit Monoclonal AntibodyMT0012
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH3C1
Western blot analysis of extracts from v5-tag fusion protein, using V5 tag antibody.
Product group Antibodies
Boster Bio
Anti-V5-Tag Rabbit Monoclonal AntibodyMT0013
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
Western blot analysis of extracts from VSV-G tag fusion protein, using VSV-G tag antibody.
Product group Antibodies
Boster Bio
Anti-VSV-G-Tag Rabbit Monoclonal AntibodyMT0017
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
Western blot analysis of p53 (acetyl K370) expression in HeLa cell lysate treated with Trichostatin A.
Product group Antibodies
Boster Bio
Anti-p53 (acetyl K370) TP53 Rabbit Monoclonal AntibodyP00001-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
Western blot analysis of p53 (acetyl K382) expression in Jurkat cell lysate treated with etopside and TSA.
Product group Antibodies
Boster Bio
Anti-p53 (acetyl K382) TP53 Rabbit Monoclonal AntibodyP00001-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
Western blot analysis of Phospho-p53 (Ser9) expression in (1)HepG2 cell lysates treated with etoposide; (2)HeLa cell lysates treated with etoposide (P00001-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody (Catalog # P00001-3) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53
Product group Antibodies
Boster Bio
Anti-Phospho-p53 (S9) TP53 Rabbit Monoclonal AntibodyP00001-3
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
Western blot analysis of Phospho-p53 (Ser33) in T47D cell lysates treated with etoposide (P00001-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody (Catalog # P00001-4) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53
Product group Antibodies
Boster Bio
Anti-Phospho-p53 (S33) TP53 Rabbit Monoclonal AntibodyP00001-4
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetTP53
Western blot analysis of Phospho-p53 (T55) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid (P00001-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody (Catalog # P00001-5) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53
Product group Antibodies
Boster Bio
Anti-Phospho-p53 (T55) TP53 Rabbit Monoclonal AntibodyP00001-5
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
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