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Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-p53 (S392) TP53 Rabbit Monoclonal AntibodyP00001-6
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid (P00001-7). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody (Catalog # P00001-7) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53
Product group Antibodies
Boster Bio
Anti-Phospho-p53 (S376) TP53 Rabbit Monoclonal AntibodyP00001-7
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal AntibodyP00003-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMTOR
Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal AntibodyP00003
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMTOR
Western blot analysis of Phospho-beta Catenin (S33/S37) expression in (1) 293T cell lysate; (2) 293T cell lysate treated with calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-beta Catenin (S33/S37) CTNNB1 Rabbit Monoclonal AntibodyP00004
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetCTNNB1
Western blot analysis of Phospho-PTEN (S380) expression in (1) MCF-7 cell treated with alkaline phosphatase lysate; (2) MCF-7 cell lysate (P00006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN monoclonal antibody (Catalog # P00006) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTEN
Product group Antibodies
References
Boster Bio
Anti-Phospho-PTEN (S380) Rabbit Monoclonal AntibodyP00006
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPTEN
Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF (P00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # P00007-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
Product group Antibodies
References
Boster Bio
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal AntibodyP00007-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.
Product group Antibodies
References
Boster Bio
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal AntibodyP00007-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin.
Product group Antibodies
Boster Bio
Anti-Phospho-ATM (S1981) Rabbit Monoclonal AntibodyP00014-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetATM
Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP (P00018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 monoclonal antibody (Catalog # P00018-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1
Product group Antibodies
Boster Bio
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal AntibodyP00018-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSIRT1
Western blot analysis of Phospho-SIRT1 (T530) expression in (1) 293T cell lysate; (2) 293T cell treated with Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-SIRT1 (T530) Rabbit Monoclonal AntibodyP00018
ApplicationsWestern Blot
ReactivityHuman
TargetSIRT1
Western blot analysis of Phospho-EGFR (Y1086) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with EGF (P00023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
Product group Antibodies
Boster Bio
Anti-Phospho-EGFR (Y1086) Rabbit Monoclonal AntibodyP00023-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetEGFR
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