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Western blot analysis of Histone H2B (acetyl K20) expression in HeLa cell treated with TSA lysate (P12718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H2BB monoclonal antibody (Catalog # P12718) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H2BB
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH2BC3
Western blot analysis of Histone H2B expression in Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate (P30930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTB1 monoclonal antibody (Catalog # P30930) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTB1
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityYeast
TargetHTB1
Western blot analysis of Phospho-Histone H2A (S129) expression in Saccharomyces cerevisiae treated with Methyl methanesulfonate.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityYeast
Western blot analysis of ABI-1 using anti-ABI-1 antibody (PA1001). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI-1 antigen affinity purified polyclonal antibody (Catalog # PA1001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI-1 at approximately 65KD. The expected band size for ABI-1 is at 55KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetABI1
Anti-Angiopoietin 2 antibody, PA1005, Western blotting Lane 1: Recombinant Human ANG2 Protein 10ng Lane 2: Recombinant Human ANG2 Protein 5ng Lane 3: Recombinant Human ANG2 Protein 2.5ng
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Human
TargetANGPT2
Anti-Annexin A1 antibody, PA1006, IHC(P) IHC(P): Human Placenta Tissue
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetANXA1
IHC analysis of Annexin IV/ANXA4 using anti-Annexin IV/ANXA4 antibody (PA1007-1).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetANXA4
Anti-Annexin V antibody, PA1008, IHC(P) IHC(P): Rat Liver Tissue
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetANXA5
IHC analysis of API5 using anti-API5 antibody (PA1009).
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetAPI5
Western blot analysis of Aquaporin 1 using anti-Aquaporin 1 antibody (PA1010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate, Lane 2: Rat Lung Tissue Lysate, Lane 3: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1 antigen affinity purified polyclonal antibody (Catalog # PA1010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 28KD. The expected band size for Aquaporin 1 is at 28KD.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetAQP1
Western blot analysis of Bax using anti-Bax antibody (PA1013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Ovary Tissue Lysate Lane 5: HELA Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: A549 Cell Lysate Lane 8: JURKAT Cell Lysate Lane 9: Human Placenta Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetBAX
Anti-BDNF antibody, PA1014, Western blotting All lanes: Anti BDNF (PA1014) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug Lane 3: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 28KD Observed bind size: 37KD
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetBDNF