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Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1) SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal AntibodyP01423-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD5
Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate (P01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 monoclonal antibody (Catalog # P01423) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5
Product group Antibodies
Boster Bio
Anti-Phospho-Smad1/5/9 (S463/S465/S467) Rabbit Monoclonal AntibodyP01423
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSMAD5
Western blot analysis of Lyn phosphorylation expression in Jurkat cell lysate treated with Pervanadate.
Product group Antibodies
Boster Bio
Anti-Phospho-Lyn (Y396) Rabbit Monoclonal AntibodyP01424-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetLYN
Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate (P01475). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # P01475) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KB1
Product group Antibodies
References
Boster Bio
Anti-Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal AntibodyP01475
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Rat
TargetRPS6KB1
Western blot analysis of Phospho-LAT (Y191) in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-LAT (Y191) Rabbit Monoclonal AntibodyP01654
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetLAT
Western blot analysis of Phospho-PKC zeta (T560) expression in HeLa cell treated with Calyculin A lysate (P01796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCZ monoclonal antibody (Catalog # P01796) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCZ
Product group Antibodies
Boster Bio
Anti-Phospho-PKC zeta (T560) PRKCZ Rabbit Monoclonal AntibodyP01796
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCZ
Western blot analysis of Phospho-PP2A alpha (Y307) in (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF.
Product group Antibodies
Boster Bio
Anti-Phospho-PP2A alpha (Y307) PPP2CA Rabbit Monoclonal AntibodyP01893
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPPP2CA
Western blot analysis of Phospho-c-Jun (T91) expression in human brain lysate (P02038-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUN monoclonal antibody (Catalog # P02038-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUN
Product group Antibodies
Boster Bio
Anti-Phospho-c-Jun (T91) Rabbit Monoclonal AntibodyP02038-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetJUN
Western blot analysis of Phospho-c-Jun (S63) expression in NIH/3T3 cell lysate treated with Anisomycin (P02038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-c-Jun (S63) monoclonal antibody (Catalog # P02038) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-c-Jun (S63) Rabbit Monoclonal AntibodyP02038
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetJUN
Western blot analysis of Phospho-RPA2 (T21) expression in HeLa cell lysate treated with Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-RPA2 (T21) Rabbit Monoclonal AntibodyP02067-1
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetRPA2
Western blot analysis of Phospho-CDC37 (S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate (P02169). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC37 monoclonal antibody (Catalog # P02169) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC37
Product group Antibodies
Boster Bio
Anti-Phospho-CDC37 (S13) Rabbit Monoclonal AntibodyP02169
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCDC37
Western blot analysis of Phospho-CrkII (Tyr221) expression in (1) K562 cell lysate treated with AP; (2) K562 cell lysate (P02533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRK monoclonal antibody (Catalog # P02533) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRK
Product group Antibodies
Boster Bio
Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal AntibodyP02533
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCRK
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