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Anti-liver FABP antibody, PA1229-1, Western blotting Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: HELA Cell Lysate Lane 4: NEURO Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFABP1
Anti-Aquaporin 5 antibody, PA1230, Western blotting Working concentration: 0.5μg/ml Lane 1: Rat Lung Tissue Lysate Lane 2: Rat Testis Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAqp5
Anti-Bcl-XS antibody, PA1232, Western blotting All lanes: Anti Bcl-XS (PA1232) at 0.5ug/ml Lane 1: HELA Whole Cell Lysate at 40ug Lane 2: PC-12 Whole Cell Lysate at 40ug Predicted bind size: 19KD Observed bind size: 19KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetBCL2L1
Anti-Cullin 4B antibody, PA1233, IHC(P) IHC(P): Zebrafish Body Tissue
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat, Zebra Fish
TargetCUL4B
Anti-PKC gamma antibody, PA1234, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Human, Mouse, Rat
TargetPRKCG
Anti-SSX2 antibody, PA1235, Western blotting Lane 1: MCF-7 Cell Lysate Lane 2: HELA Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSSX2
IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetCXCR4
Western blot analysis of GFAP using anti-GFAP antibody (PA1239). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Mouse Brain Tissue Lysate, Lane 3: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PA1239) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 49 KD. The expected band size for GFAP is at 49 KD.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetGFAP
Anti-Cytokeratin 8 antibody, PA1240, Western blotting Lane 1: HELA Cell Lysate Lane 2: HT1080 Cell Lysate Lane 3: COLO320 Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetKRT8
Anti-TNF Receptor II antibody, PA1243, Western blotting Lane 1: MM453 Cell Lysate Lane 2: JURKAT Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityCanine, Human
TargetTNFRSF1B
Anti-Cyclin D1 antibody, PA1245-1, Western blotting Lane 1: Rat Testis Tissue Lysate Lane 2: Rat Ovary Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: HELA Cell Lysate Lane 5: MM231 Cell Lysate Lane 6: SW620 Cell Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetCcnd1
Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (PA1245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Human Placenta Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: MCF-7 Whole Cell Lysate, Lane 5: COLO320 Whole Cell Lysate, Lane 6: SW620 Whole Cell Lysate, Lane 7: MM231 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # PA1245-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 33KD. The expected band size for Cyclin D1 is at 33KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot
ReactivityChicken, Human, Rat
TargetCCND1