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Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody, PA1203, Western blotting WB: Rat Skeletal Muscle Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetCHRNA1
Western blot analysis of HRH3 using anti-HRH3 antibody (PA1204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates Lane 2: human SHG-44 whole cell lysates Lane 3: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified polyclonal antibody (Catalog # PA1204) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 49KD. The expected band size for HRH3 is at 49KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Human, Mouse, Rat
TargetHRH3
Anti-MRGX1 antibody, PA1208, Western blotting Lane 1: Marker Lane 2: Rat Heart Tissue Lysate Lane 3: Rat Skeletal Muscle Tissue Lysate Lane 4: MCF-7 Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityRat
TargetMrgprx1
Anti-TNF Receptor I antibody, PA1210, IHC(P) IHC(P): Human Mammary Tissue
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTNFRSF1A
Anti-beta Catenin antibody, PA1212-1, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Heart Tissue Lysate Lane 3: Rat Testis Tissue Lysate Lane 4: MCF-7 Cell Lysate Lane 5: HELA Cell Lysate Lane 6: M453 Cell Lysate Lane 7: M231 Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetCTNNB1
Anti-beta Catenin antibody, PA1212, Western blotting Lane 1: MM453 Cell Lysate Lane 2: MCF-7 Cell Lysate Lane 3: HELA Cell Lysate
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetCTNNB1
IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetHSPA1A
Anti-Notch1 antibody, PA1215, Western blotting All lanes: Anti Notch1 (PA1215) at 0.5ug/ml Lane 1: MCF-7 Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: JURKAT Whole Cell Lysate at 40ug Lane 4: COLO320 Whole Cell Lysate at 40ug Predicted bind size: 272KD Observed bind size: 272KD
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human
TargetNOTCH1
Anti-FGF8 antibody, PA1216, Western blotting WB: Rat Ovary Tissue Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetFGF8
Anti-XAF1 antibody, PA1218, IHC(P) IHC(P): Human Endometrial Carcinoma Tissue
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetXAF1
Anti-NMDAR1 antibody, PA1222, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: Rat Liver Tissue Lysate Lane 4: Rat Heart Tissue Lysate Lane 5: MM453 Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: HELA Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetGRIN1
Western blot analysis of RUNX2 using anti-RUNX2 antibody (PA1224). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PA1224) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 62KD. The expected band size for RUNX2 is at 58KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetRUNX2