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Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAIMP2
Anti-Tau antibody, PA1482, Western blotting All lanes: Anti (PA1482) at 0.5ug/ml Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug Lane 3: HT1080 Whole Cell Lysate at 40ug Lane 4: MCF-7 Whole Cell Lysate at 40ug Predicted bind size: 79KD Observed bind size: 79KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAPT
Anti-MTA1 antibody, PA1483, IHC(F) IHC(F): Rat Ovary Tissue
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetMTA1
Anti-TLR4 antibody, PA1484, Western blotting Lane 1: HELA Cell Lysate Lane 2: SMMC Cell Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTLR4
Anti-ANGPTL1 antibody, PA1487, Western blotting Lane 1: A549 Cell Lysate Lane 2: SW620 Cell Lysate Lane 3: MCF-7 Cell Lysate Lane 4: MM231 Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetANGPTL1
Western blot analysis of AQP3 using anti-AQP3 antibody (PA1488).
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetAQP3
Anti-GPR2/CCR10 antibody, PA1491, Western blotting Lane 1: HELA Cell Lysate Lane 2: SW620 Cell Lysate Lane 3: A549 Cell Lysate Lane 4: MM231 Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetCCR10
Anti-GCLC antibody, PA1492, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Heart Tissue Lysate Lane 4: Rat Lung Tissue Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetGCLC
Anti-IFN gamma antibody, PA1493, Western blotting All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml Lane 1: Recombinant Human IFN gamma Protein 10ng Lane 2: Recombinant Human IFN gamma Protein 5ng Lane 3: Recombinant Human IFN gamma Protein 2.5ng Lane 4: Recombinant Human IFN gamma Protein 1.25ng Predicted bind size: 19KD Observed bind size: 19KD
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Human
TargetIFNG
IHC analysis of APEX1 using anti-APEX1 antibody (PA1494).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetAPEX1
Anti-CAMKK2 antibody, PA1497, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: Mouse Brain Tissue Lysate Lane 4: Mouse Brain Tissue Lysate
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Mouse, Rat
TargetCAMKK2
Anti-IGFBP-3 antibody, PA1498, Western blotting All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml Lane 1: 293T Whole Cell Lysate at 40ug Lane 2: MCF-7 Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: SW620 Whole Cell Lysate at 40ug Predicted bind size: 32KD Observed bind size: 40KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Rat
TargetIGFBP3