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Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
Anti-MEKK2/MAP3K2 Antibody Picoband(r)PA1499
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
Anti-Apelin antibody, PA1501, Western blotting Working concentration of primary antibody: 0.5 μg/ml; 40μg protein was loaded. Lane 1: U87 Cell Lysate Lane 2: MCF-7 Cell Lysate Lane 3: HELA Cell Lysate Lane 4: MM453 Cell Lysate
Product group Antibodies
Boster Bio
Anti-Apelin/APLN Antibody Picoband(r)PA1501
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetAPLN
Anti-BCAT2 antibody, PA1502, Western blotting All lanes: Anti BCAT2 (PA1502) at 0.5ug/ml
Product group Antibodies
Boster Bio
Anti-BCAT2 Antibody Picoband(r)PA1502
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetBCAT2
Anti-MCL1 antibody, PA1503, Western blotting All lanes: Anti MCL1 (PA1503) at 0.5ug/ml
Product group Antibodies
Boster Bio
Anti-MCL1 Antibody Picoband(r)PA1503
ApplicationsWestern Blot
ReactivityHamster, Human
TargetMCL1
Anti-Bcl10 antibody, PA1504, Western blotting Lane 1: U87 Cell Lysate Lane 2: MCF-7 Cell Lysate Lane 3: HELA Cell Lysate Lane 4: COLO320 Cell Lysate
Product group Antibodies
Boster Bio
Anti-B-cell lymphoma/leukemia 10 Bcl10 Antibody Picoband(r)PA1504
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetBCL10
Anti-Bone Sialoprotein antibody, PA1505, IHC(P) IHC(P): Human Osteosarcoma Tissue
Product group Antibodies
References
Boster Bio
Anti-Bone Sialoprotein/IBSP Antibody Picoband(r)PA1505
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetIBSP
Anti-NSE antibody, PA1061, Western blotting WB: Rat Brain Tissue Lysate
Product group Antibodies
Boster Bio
Anti-NSE/ENO2 Antibody Picoband(r)PA1061
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetENO2
Anti-Neurotrophin 3 antibody, PA1062, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HELA Cell Lysate
Product group Antibodies
References
Boster Bio
Anti-Neurotrophin 3/NTF3 Antibody Picoband(r)PA1062
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetNTF3
IHC analysis of PKC alpha using anti-PKC alpha antibody (PA1065).
Product group Antibodies
Boster Bio
Anti-PKC alpha/PRKCA Antibody Picoband(r)PA1065
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPRKCA
Anti-PMVK antibody, PA1067, Western blotting WB: Rat Cardiac Muscle Tissue Lysate
Product group Antibodies
Boster Bio
Anti-Phosphomevalonate kinase PMVK Antibody Picoband(r)PA1067
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPMVK
IHC analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068).
Product group Antibodies
Boster Bio
Anti-PP2A-alpha/PPP2CA Antibody Picoband(r)PA1068
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetPPP2CA
Anti-RAGE antibody, PA1069, IHC(P) IHC(P): Rat Lung Tissue
Product group Antibodies
References
Boster Bio
Anti-RAGE/AGER Antibody Picoband(r)PA1069
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHamster, Human, Mouse, Rat
TargetAGER
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