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Flow Cytometry: Analysis of PSMA expression on on LNCaP cells (Right) and Jurkat Cells (Left). Open histograms indicate the reaction of isotypic control to the cells. Shaded histograms indicate the reaction of AM20199RP-N PSMA antibody to the cells.
Product group Antibodies
OriGene
PSMA (FOLH1) Mouse Monoclonal Antibody [Clone ID: 107-1A4]AM20199RP-N
ApplicationsFlow Cytometry
ReactivityHuman
TargetFOLH1
  • SizePrice
Product group Antibodies
OriGene
C Reactive Protein (CRP) Mouse Monoclonal Antibody [Clone ID: C5]AM20202PU-N
ApplicationsELISA
ReactivityHuman
TargetCRP
  • SizePrice
Product group Antibodies
OriGene
C Reactive Protein (CRP) Mouse Monoclonal Antibody [Clone ID: C2]AM20203PU-N
ApplicationsELISA
ReactivityHuman
TargetCRP
  • SizePrice
Monoclonal antibody ATG5-7C6 detects the endogenous Atg5 as well as the overexpressed 33 kDa-protein in Jurkat cells. Cells were fixed with 4% paraformaldehyd for 5 min and permeabilized with 0.05% Saponin. After 10 min aceton-treatment, Atg5 was detected with mab ATG5-7C6 at 0.5 ug/ml. Images by courtesy of Hans-Uwe Simon, MD, PhD, University of Bern.
Product group Antibodies
OriGene
ATG5 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7C6]AM20205PU-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityCanine, Human, Mouse, Rat
TargetATG5
  • SizePrice
Whole lysate of serum starved SH-SY5Y cells was applied to SDS-PAGE and tranferred to a PVDF membrane. The Immunoblot was probed with the indicated monoclonal antibodies at 0.5 ug/ml for 1h at 15-22°C and developed with ECL (exposure time: 30 sec). Lane M: Molecular Weight marker Lane 1: AM20205PU-N (Clone 7C6) Lane 2: AM20206PU-N (Clone 11C3)
Product group Antibodies
OriGene
ATG5 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C3]AM20206PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetATG5
  • SizePrice
Detection of endogenous Aurora-A: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal antibody AM20207PU-N (7F12) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
Aurora A (AURKA) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]AM20207PU-N
ApplicationsWestern Blot
ReactivityCanine, Human, Mouse, Rat
TargetAURKA
  • SizePrice
Detection of endogenous Aurora-A/B: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal Aurora-A/B antibody AM20208PU-N (5F11) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Product group Antibodies
OriGene
Aurora A (AURKA) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F11]AM20208PU-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
ReactivityHuman
TargetAURKA
  • SizePrice
Antibody Sensitivity: Biotinylated Molecular Weight Marker (Biorad) was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with Biotin antibody AM20209PU-N (Clone 2H2) at 0.5 g/ml for 1h at RT and developed by ECL (exp. time:
Product group Antibodies
OriGene
Biotin Mouse Monoclonal Antibody [Clone ID: 2H2]AM20209PU-N
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
Brk (PTK6) Mouse Monoclonal Antibody [Clone ID: 24B7]AM20211PU-N
ApplicationsWestern Blot
ReactivityHuman
TargetPTK6
  • SizePrice
Product group Antibodies
OriGene
LC3B (MAP1LC3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F10]AM20212BT-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Hamster, Human, Mouse, Rat
TargetMAP1LC3B
  • SizePrice
Endogenous LC-3 punctae detected with AM20212PU-N LC3 antibody (Clone 5F10): The majority of LC-3 was diffusely localized in unstimulated COS-7 cells, wheres punctated signals of LC-3 increase after induction of autophagy by vinblastin stimulation for 2 hr. Cells were fixed with paraformaldehyd followed by methanol treatment. Cells were permeabilized with 0.3% TritonX100. Endogenous LC-3 was detected with mab 5F10. Images by courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK.
Product group Antibodies
OriGene
LC3B (MAP1LC3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5F10]AM20212PU-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityCanine, Hamster, Human, Mouse, Rat
TargetMAP1LC3B
  • SizePrice
Detection of endogenous LC-3: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with LC3 antibody (Clone: 2G6) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT Lane 8: Neuro2A Lane 9: NIH-3T3
Product group Antibodies
OriGene
LC3B (MAP1LC3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2G6]AM20213PU-N
ApplicationsImmunoFluorescence, Western Blot
ReactivityHamster, Human, Monkey, Mouse, Rat
TargetMAP1LC3B
  • SizePrice
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