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ProductsBack/MASTL (5C3) Monoclonal Antibody

MASTL (5C3) Monoclonal Antibody

BSM-51071M
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetMASTL
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Overview

  • Supplier
    Bioss
  • Product Name
    MASTL (5C3) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C3
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID84930
  • Target name
    MASTL
  • Target description
    microtubule associated serine/threonine kinase like
  • Target synonyms
    GREATWALL, GW, GWL, MAST-L, THC2, serine/threonine-protein kinase greatwall, greatwall kinase homolog, greatwall protein kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96GX5
  • Protein Name
    Serine/threonine-protein kinase greatwall
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GWL/MASTL using anti-GWL/MASTL antibody (A04567-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GWL/MASTL antigen affinity purified polyclonal antibody (Catalog # A04567-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GWL/MASTL at approximately 110 kDa. The expected band size for GWL/MASTL is at 97 kDa.
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