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Western Blot analysis of HepG2 cells using THC2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using THC2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using THC2 Polyclonal Antibody

MASTL Antibody

CSB-PA004288
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMASTL
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Overview

  • Supplier
    Cusabio
  • Product Name
    MASTL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84930
  • Target name
    MASTL
  • Target description
    microtubule associated serine/threonine kinase like
  • Target synonyms
    GREATWALL; greatwall kinase homolog; greatwall protein kinase; GW; GWL; MAST-L; serine/threonine-protein kinase greatwall; THC2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GX5
  • Protein Name
    Serine/threonine-protein kinase greatwall
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GWL/MASTL using anti-GWL/MASTL antibody (A04567-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GWL/MASTL antigen affinity purified polyclonal antibody (Catalog # A04567-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GWL/MASTL at approximately 110 kDa. The expected band size for GWL/MASTL is at 97 kDa.
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