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ProductsBack/MATK Mouse Monoclonal Antibody [Clone ID: 9D7]
Western blot analysis using MATK mouse mAb against K562 cell lysate (1).
Western blot analysis using MATK mouse mAb against K562 cell lysate (1).
Western blot analysis using MATK mouse mAb against K562 cell lysate (1).

MATK Mouse Monoclonal Antibody [Clone ID: 9D7]

AM06369PU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMATK
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MATK Mouse Monoclonal Antibody [Clone ID: 9D7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9D7
  • Gene ID4145
  • Target name
    MATK
  • Target description
    megakaryocyte-associated tyrosine kinase
  • Target synonyms
    CHK, CTK, HHYLTK, HYL, HYLTK, Lsk, megakaryocyte-associated tyrosine-protein kinase, CSK homologous kinase, Csk-homologous kinase, Csk-type protein tyrosine kinase, HYL tyrosine kinase, hematopoietic consensus tyrosine-lacking kinase, hydroxyaryl-protein kinase, leukocyte carboxyl-terminal src kinase related, protein kinase HYL, tyrosine kinase MATK, tyrosine-protein kinase CTK, tyrosylprotein kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP42679
  • Protein Name
    Megakaryocyte-associated tyrosine-protein kinase
  • Scientific Description
    MATK mouse monoclonal antibody, clone 9D7, Aff - Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MATK using anti-MATK antibody (A02050-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (Catalog # A02050-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.
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