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ProductsBack/MAX (phospho Ser11) antibody
ICC/IF analysis of U2OS cells using GTX55452 MAX (phospho Ser11) antibody. Blue : DAPI
ICC/IF analysis of U2OS cells using GTX55452 MAX (phospho Ser11) antibody. Blue : DAPI
ICC/IF analysis of U2OS cells using GTX55452 MAX (phospho Ser11) antibody. Blue : DAPI

MAX (phospho Ser11) antibody

GTX55452
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetMAX
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Overview

  • Supplier
    GeneTex
  • Product Name
    MAX (phospho Ser11) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4149
  • Target name
    MAX
  • Target description
    MYC associated factor X
  • Target synonyms
    bHLHd4; class D basic helix-loop-helix protein 4; protein max
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61244
  • Protein Name
    Protein max
  • Scientific Description
    The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAX using anti-MAX antibody (A03239). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAX antigen affinity purified polyclonal antibody (Catalog # A03239) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAX at approximately 19-21 kDa. The expected band size for MAX is at 18 kDa.
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